Globotriaosylceramide-related biomarkers of fabry disease identified in plasma by high-performance thin-layer chromatography - densitometry- mass spectrometry

High performance thin-layer chromatography and densitometry of synaptic plasma membrane lipids.

Previously, it has been shown that phospholipids, cholesterol, and glycolipids could be quantitated using the same high performance thin-layer chromatography (HPTLC) method. Here we examined that method in terms of linearity of standards in the nanogram range, recovery of nonacidic and acidic lipids after Sephadex column chromatography, and quantitation of lipids in mouse synaptic plasma membra...

Speciation of chromium by high-performance thin-layer chromatography with direct determination by laser ablation inductively coupled plasma mass spectrometry.

It is of considerable importance to be able to distinguish metallic species because their toxicity depends on their chemical form. Therefore, the analysis of environmental samples can be enhanced by the combination of high-performance thin-layer chromatography (HPTLC) with laser ablation inductively coupled plasma mass spectrometry (LA-ICPMS). In this study, Cr (3+) and Cr (6+) were separated o...

Trace analysis of acrylamide by high-performance thin-layer chromatography coupled to mass spectrometry

Determination of oxycodone hydrochloride in oral solutions by high-performance thin-layer chromatography/densitometry.

A thin-layer chromatography/densitometry method was developed and validated for the determination of oxycodone hydrochloride in oral solutions by using silica gel plates. A horizontal technique was used for development of the plates. The optimum composition for the mobile phase, which provided a suitable Rf value of 0.6 for oxycodone, was propanol-acetic acid-water-25% ammonia-methanol (20 + 1 ...

Determination of gangliosides in human cerebrospinal fluid by high-performance thin-layer chromatography and direct densitometry.

A method for the separation and quantification of a complex ganglioside mixture from a clinically available amount (5 ml) of human cerebrospinal fluid (CSF) is described. After reduction of the CSF volume by ultrafiltration, gangliosides are extracted with methanol/chloroform, then separated and quantified by high performance thin layer chromatography (HPTLC) and direct densitometry. For purifi...